Publications

McCracken NA, Liu H, Runnebohm AM, Wijeratne HRS, Wijeratne AB, Staschke KA, Mosley AL. Obtaining Functional Proteomics Insights from Thermal Proteome Profiling through Optimized Melt Shift Calculation and Statistical Analysis with InflectSSP. Mol Cell Pro, 2023. 10.1016/j.mcpro.2023.100630

McCracken NA, Justice SAP, Wijeratne AB, Mosley AL. Inflect: Optimizing Computational Workflows for Thermal Proteome Profiling Data Analysis. J Proteome Res, 2021. 20(4):1874-1888. 10.1021/acs.jproteome.0c00872

Justice SAP, McCracken NA, Victorino JF, Qi GD, Wijeratne AB, Mosley AL. Boosting Detection of Low-Abundance Proteins in Thermal Proteome Profiling Experiments by Addition of an Isobaric Trigger Channel to TMT Multiplexes. Anal Chem, 2021. 93(18): 7000-7010. 10.1021/acs.analchem.1c00012

Justice SAP, Barron MP, Qi GD, Wijeratne HRS, Victorino JF, Simpson ER, Vilseck JZ, Wijeratne AB, Mosley AL. Mutant thermal proteome profiling for characterization of missense protein variants and their associated phenotypes within the proteome. J Biol Chem, 2020. 295(48): 16219-16238. 10.1074/jbc.RA120.014576

Victorino JF, Fox MJ, Smith-Kinnaman WR, Peck Justice SA, Burriss KH, et al. RNA Polymerase II CTD phosphatase Rtr1 fine-tunes transcription termination. PLOS Genetics, 2020. 16(3): e1008317

Sarah A. Peck Justice, Guihong Qi, H. R. Sagara Wijeratne, José F. Victorino, Ed R. Simpson, Aruna B. Wijeratne, Amber L. Mosley. Temperature sensitive Mutant Proteome Profiling: a novel tool for the characterization of the global impact of missense mutations on the proteome. BioRxiv, 2019.

Levasseur, E.M., K. Yamada, A.R. Piñeros, W. Wu, F. Syed, K.S. Orr, E. Anderson-Baucum, T.L. Mastracci, B. Maier, A.L. Mosley, Y. Liu, E. Bernal-Mizrachi, L.C. Alonso, D. Scott, A. Garcia-Ocaña, S.A. Tersey, and R.G. Mirmira, Hypusine biosynthesis in β cells links polyamine metabolism to facultative cellular proliferation to maintain glucose homeostasis. Science Signaling, 2019. 12(610): p. eaax0715.

Harris, M.T., V. Jeffers, J. Martynowicz, J.D. True, A.L. Mosley, and W.J. Sullivan, A novel GCN5b lysine acetyltransferase complex associates with distinct transcription factors in the protozoan parasite Toxoplasma gondii. Molecular and Biochemical Parasitology, 2019. 232: p. 111203.

Burriss, K.H. and A.L. Mosley, Methods review: Mass spectrometry analysis of RNAPII complexes. Methods, 2019. 159-160: p. 105-114.

Peck, S.A., K.D. Hughes, J.F. Victorino, and A.L. Mosley, Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control. WIREs RNA, 2019. 10(4): p. e1529.

Shao, Y., E. Wichern, P.J. Childress, M. Adaway, J. Misra, A. Klunk, D.B. Burr, R.C. Wek, A.L. Mosley, Y. Liu, A.G. Robling, N. Brustovetsky, J. Hamilton, K. Jacobs, D. Vashishth, K.R. Stayrook, M.R. Allen, J.M. Wallace, and J.P. Bidwell, Loss of Nmp4 optimizes osteogenic metabolism and secretion to enhance bone quality. American Journal of Physiology-Endocrinology and Metabolism, 2019. 316(5): p. E749-E772.

Yamamoto, W.R., R.N. Bone, P. Sohn, F. Syed, C.A. Reissaus, A.L. Mosley, A.B. Wijeratne, J.D. True, X. Tong, T. Kono, and C. Evans-Molina, Endoplasmic reticulum stress alters ryanodine receptor function in the murine pancreatic beta cell. J Biol Chem, 2019. 294(1): p. 168-181.

Fox, M.J., J.F. Victorino, W.R. Smith-Kinnaman, S.A.P. Justice, H. Gao, A.K. Boyd, M.A. Zimmerly, R.R. Chan, G.O. Hunter, Y. Liu, and A.L. Mosley, RNA Polymerase II CTD phosphatase Rtr1 prevents premature transcription termination. bioRxiv, 2019: p. 708727.

Dronamraju, R., J.L. Kerschner, S.A. Peck, A.J. Hepperla, A.T. Adams, K.D. Hughes, S. Aslam, A.R. Yoblinski, I.J. Davis, A.L. Mosley, and B.D. Strahl, Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction. Cell Reports, 2018. 25(12): p. 3476-3489.e5.

Watkins, D.S., J.D. True, A.L. Mosley, and A.J. Baucum, 2nd, Proteomic Analysis of the Spinophilin Interactome in Rodent Striatum Following Psychostimulant Sensitization. Proteomes, 2018. 6(4).

Gökmen-Polar, Y., J.D. True, E. Vieth, Y. Gu, X. Gu, G.D. Qi, A.L. Mosley, and S.S. Badve, Quantitative phosphoproteomic analysis identifies novel functional pathways of tumor suppressor DLC1 in estrogen receptor positive breast cancer. PLOS ONE, 2018. 13(10): p. e0204658.

Willy, J.A., S.K. Young, A.L. Mosley, S. Gawrieh, J.L. Stevens, H.C. Masuoka, and R.C. Wek, Function of inhibitor of Bruton's tyrosine kinase isoform alpha (IBTKalpha) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response. J Biol Chem, 2017. 292(34): p. 14050-14065.

Hunter, G.O., Fox, M.J., Smith-Kinnaman, W.R., Gogol, M., Fleharty, B., Mosley, A.L. Phosphatase Rtr1 Regulates Global Levels of Serine 5 RNA Polymerase II C-Terminal Domain Phosphorylation and Cotranscriptional Histone Methylation. Molecular and Cellular Biology. 2016 Aug 12;36(17):2236-45. doi: 10.1128/MCB.00870-15. PMID:27247267

Bedard, L.G., Dronamraju, R., Kerschner, J.L., Hunter, G.O., Axley, E.D., Boyd, A.K., Strahl, B.D., Mosley, A.L. Quantitative analysis of dynamic protein interactions during transcription reveals a role for casein kinase II in PAF complex phosphorylation and regulation of H2B monoubiquitylation. Journal of Biological Chemistry. 2016 Jun 24;291(26):13410-20. doi: 10.1074/jbc.M116.727735. PMID: 27143358

Fox, M.J., Mosley, A.L. Rrp6: Integrated roles in nuclear RNA metabolism and transcription termination. WIREs RNA. 2016 Jan-Feb;7(1):91-104. doi: 10.1002/wrna.1317. Epub 2015 Nov 26. Review.

Simon, J.M., Parker, J.S., Liu, F., Rothbart, S.B., Ait-Si-Ali, S., Strahl, B.D., Jin, J., Davis, I.J., Mosley, A.L., Pattenden, S.G. A Role for Widely Interspaced Zinc Finger (WIZ) in Retention of the G9a Methyltransferase on Chromatin. Journal of Biological Chemistry. 2015 Oct 23;290(43):26088-102. doi: 10.1074/jbc.M115.654459. Epub 2015 Sep 3.

Maganti, A.V., Maier, B.F. , Tersey, S.A., Sampley, M.L., Mosley, A.L., Ozcan, S., Pachaiyappan, B., Woster, P.M., Hunter, C.S., Stein, R., Mirmira, R.G. Transcriptional Activity of the Islet beta Cell Factor Pdx1 is Augmented by Lysine Methylation Catalyzed by the Methyltransferase Set7/9. Journal of Biological Chemistry. 2015 Feb 24. pii: jbc.M114.616219. Epub 02/2015.

Fox, M.J., Gao, H., Smith-Kinnaman, W. R., Liu, Y., Mosley, A.L. The Exosome Component Rrp6 Is Required for RNA Polymerase II Termination at Specific Targets of the Nrd1-Nab3 Pathway. 02/2015. PLoS Genetics. 10(2): e1004999.

Zhang, Q., Zhou, X., Wu, R., Mosley, A., Zeng, S.X., Xing, Z., Lu, H. The role of IMP dehydrogenase 2 in Inauhzin-induced ribosomal stress. eLife. 2014 Oct 27;3.

Ni, Z. Xu, C. Guo, X. Hunter, G.O., Kuznetsova, O., Tempel, W., Marcon, E., Zhong, G., Guo, H., Kuo, W., Li, J., Young, P., Olsen, J.B., Wan, C., Loppnau, P., Bakkouri, M.E., Senisterra, G., He, H., Huang, H-M., Sidhu, S., Emili, A., Murphy, S., Mosley, A.L., Arrowsmith, C., Min, J., Greeblatt, J.F. RPRD1A and RPRD1B serve as RNA Polymerase II Carboxy-Terminal Domain Scaffolds to Recruit RPAP2 for Serine 5 Dephosphorylation. Nature Structural & Molecular Biology. 2014 Aug;21(8):686-95. doi: 10.1038/nsmb.2853.

Hsu, P.L., Yang, F., Smith-Kinnaman, W.R., Yang, W., Zheng, N., Mosley, A.L., Varani, G. Rtr1 is a dual specificity phosphatase that dephosphorylates Tyr1 and Ser5 on the RNA Polymerase II CTD. Journal of Molecular Biology. 2014 Aug 12;426(16):2970-81. doi: 10.1016/j.jmb.2014.06.010.

Smith-Kinnaman, W.R., Berna, M.J., Hunter, G.O., True, J.D., Hsu, P., Cabello, G.I., Fox, M.J., Varani, G., Mosley, A.L. The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. Molecular Biosystems. 2014 Jul;10(7):1730-41.

Khanna, M., Imasaki, I., Chikwana, V.M., Peres-Miller, S., Hunter, G.O., Mosley, A.L., Takagi, Y.,Hurley, T.D. Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells. Protein Expr Purif. 2013 Aug;90(2):78-83. doi: 10.1016/j.pep.2013.05.007. Epub 2013 May 24.

Mosley, A.L. *, Hunter, G.O., Sardiu, M., Smolle, M.E., Workman, J.L., Florens, L., Washburn, M.P.* Quantitative Proteomics Demonstrates that the RNA Polymerase II Subunits Rpb4 and Rpb7 Dissociate During Transcription Elongation. Molecular & Cellular Proteomics. 2013 Jun;12(6):1530-8. doi: 10.1074/mcp.M112.024034. Epub 2013 Feb 15. * = co-corresponding authors.

Chen, X.F., Lehmann, L., Lin, J.J., Vashisht, A., Schmidt, R., Ferrari, R., Huang, C., McKee, R., Mosley, A., Plath, K., Kurdistani, S.K., Wohlschlegel, J., Carey, M. Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function. Cell Reports. 2012 Nov 29;2(5):1061-7. doi: 10.1016/j.celrep.2012.10.019. Epub 2012 Nov 21.

Zhang, D.W., Mosley, A.L., Ramisetty, S.R., Rodriguez-Molina, J.B., Washburn, M.P., Ansari, A.Z. Ssu72 phosphatase dependent erasure of phospho-Ser7 marks on the RNA Polymerase II C-terminal domain is essential for viability and transcription termination. Journal of Biological Chemistry. 2012 Mar 9; 287(11):8541-8551.

Daniels, D., Mendez, J. Mosley, A.L. Ramisetty, S., Murphy, N., Benink, H. Wood, K., Urh, M., and Washburn, M.P. Examining the Complexity of Human RNA Polymerase Complexes using HaloTag Technology Coupled to Label Free Quantitative Proteomics. Journal of Proteome Research. 2012 Feb 3; 11(2):564-575.

Mosley, A.L., Sardiu, M.E., Pattenden, S.G., Workman, J.L, Florens, L., and Washburn, M.P. Highly reproducible label free quantitative proteomic analysis of RNA polymerase complexes. Molecular & Cellular Proteomics. 2011 Feb. 10(2): M110.000687.

Fournier, M.L., Paulson, A., Pavelka, N., Mosley, A.L., Gaudenz, K., Bradford, W.D., Glynn, E., Li, H., Sardiu, M.E., Fleharty, B., Seidel, C., Florens, L., Washburn, M.P. Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin. Molecular & Cellular Proteomics. 2010 Feb. 9(2):271-284.

Mosley, A.L., Pattenden, S.G., Carey, M., Venkatesh, S., Gilmore, J.M., Florens, L., Workman, J.L., Washburn, M.P. Rtr1 is a CTD phosphatase that regulates RNA polymerase II during the transition from serine 5 to serine 2 phosphorylation. Molecular Cell. 2009. 34(2):168-178.

Mosley, A.L., Florens, L., Wen, Z., Washburn, M.P. A label free quantitative proteomic analysis of the Saccharomyces cerevisiae nucleus. Journal of Proteomics. 2009 Feb 15. 72(1):110-20.

Black, J.C., Mosley, A., Kitada, T. Washburn, M.P., Carey, M. The SIRT2 deacetylase regulates autoacetylation of p300. Molecular Cell. 2008. 32(3):449-455.

Zybailov, B.L., Mosley, A.L., Sardiu, M.E., Coleman, M.K., Florens, L., and Washburn, M.P. Statistical Analysis of Membrane Proteome Expression Changes in Saccharomyces cerevisiae. Journal of Proteome Research. 2006 Sep. 5(9):2339-47.

Finlin, B.S., Mosley, A.L., Crump, S.M., Correll, R.N., Ozcan, S., Satin, J., Andres, D.A. Regulation of L-type Ca2+ channel activity and insulin secretion by the Rem2 GTPase. Journal of Biological Chemistry. 2005. 280(51):41864-41871.

Mosley, A. L. and Özcan, S. Pdx-1 Interacts with Histone Deacetylases Hdac-1 and Hdac-2 on Low Levels of Glucose. Journal of Biological Chemistry. 2004. 279(52):54241-54247.

Mosley, A. L. and Özcan, S. Glucose regulation of insulin gene expression requires the recruitment of p300 by the beta-cell specific transcription factor Pdx-1. Molecular Endocrinology. 2004. 18(9):2279-2290.

Lakshmanan, J.*, Mosley, A.L.*, and Özcan, S. Std1 and Mth1 are Required for Repression of Transcription by Rgt1 in the Absence of Glucose. Current Genetics. 2003. 44: 19-25. * = co-first author.

Mosley, A.L. and Özcan, S. Glucose Regulates Insulin Gene Transcription by Hyperacetylation of Histone H4. Journal of Biological Chemistry. 2003. 278(22): 19660-19666

Mosley, A.L.*, Lakshmanan, J.*, Aryal, B., Özcan, S. Glucose-Mediated Phosphorylation Converts the Transcription Factor Rgt1 from a Repressor to an Activator. Journal of Biological Chemistry. 2003. 278(12): 10322-10327. * = co-first author.

Özcan, S., Mosley, A.L., and Aryal, B. Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast. BBRC. 2002. 295(3):724-729.